La citologia, uno studio di biologia cellulare, si concentra sull'interazione della luce e dei campioni in un microscopio ottico. Questo diagramma illustra come la luce deviata e non deviata interagiscono per creare immagini di strutture cellulari. Il diagramma aiuta a comprendere i processi di imaging cellulare.
1646 x 1518 px | 27,9 x 25,7 cm | 11 x 10,1 inches | 150dpi
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. Cytology. Cytology. over the entire field of view; the deviated fight tends to focus at the plane of the eyepiece lens (Figure 11-12). The image of the object is formed as a result of interference between the deviated and undeviated light coming from the object. The image of its surroundings is formed by the undeviated light transmitted by this component only. Separation of the deviated and undeviated light occurs in the optical microscope at the rear focal plane of the objective.. Specimen Figure 11-11. Diagram Showing the Diffraction of Light on Passing Through and Around the Edges of an Inhomogeneous Object. Light deviated from its original path by the object is spread over the entire surface of the objective lens, while the light transmitted undeviated by the object passes through the more central part of this same lens. The optical system of the phase-contrast microscope differs from that of the ordinary optical microscope only in the addition of (1) a substage annular diaphragm to illuminate the object with a narrow cone of light, and (2) a diffraction plate mounted in the objective (Figure 11-13). The diffraction plate is located at the rear focal plane of the objective where the deviated and undeviated light coming from the object is sepa- rated. The relative phase of the deviated and undeviated light is changed by introducing a layer of phase-retarding material on that part of the diffraction plate covered by either one of these rays. As already pointed out, the deviated light originating at the object is retarded or altered in phase (about V4 wavelength) with respect to the light transmitted un- deviated by the object. By further retarding this deviated light by another V4 wavelength at the diffraction plate, it wiU have a total retardation of Vi wavelength and will be completely out of phase with that of the undeviated light (Figure 11-13). The deviated and undeviated light SURVEY OF CYTOLOGICAL TECHNIQUES / 229. Please note that these images are extrac
